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KMID : 0382419990250010117
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Korean Journal of Environmental Health Society
1999 Volume.25 No. 1 p.117 ~ p.124
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A Study on Cellular Toxicity of Mercurials and Protection of Selenium against Mercurials in RAW264.7 Cell
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Koh Dai-Ha
Youm Jung-Ho
Kim Jun-Youn
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Abstract
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In present study, we elucidated the protective effects of selenium against the cytotoxicity of mercurial compounds(CH_3HgCl, HgCl_2) in RAW 264.7 cell and peritoneal macrophages from Balb/c mice. Mercurial compounds significantly decreased the cell viability and the synthesis of nitric oxide(NO) and cellular ATP in a dose-dependent manner. Selenium was not toxic at concentrations of 1-10 μM. But, selenium by itself was decreased the cell viability and the syntheses of NO and cellular ATP in dose-dependent manner above 20 μM conditions. However, in the selenium added conditions, the mercurial-induced cytotocixity was not observed. The protective effects of selenium against the cytotoxicity of mercurial compound depended on the concentration of added selenium to the culture conditions, We evaluated the mechanisms of protective effect of selenium against the mercury-induced cytotoxicity by the pretreatment experiments. In the selenium pretreatment, mercurial compounds still decreased the cell viability and the syntheses of NO and cellular ATP in a dose-dependent manner. These results indicated that the protective effect of selenium against the cytotoxicity of mercurial compounds was achieved by formation of some complex as such as Se-Hg or Se-Hg-protein. Though its mechanism was not clear, the protective role of selemium against the mercury toxicity would be exhibited in the immunological system.
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